The position is placed at the Faculty of Health and Society, Department of Biomedical Science. The education and research activities comprised by the faculty aim at contributing to the knowledge base in order to preserve, support and promote people’s physical, psychological, and social health. Research is taking place in areas ranging from science, technology and medicine to social science. At the interfaces between them, exciting new areas for research are being generated. The research at the faculty is also linked to the research programme Biofilms - Research Center for Biointerfaces which combines research with medical, dental, food and environmental applications, at three of our faculties, in close collaboration with regional industry.
PhD-student position in Biomedical science
In order to be considered for appointment as a doctoral student, the applicant must meet the general entry requirements for third-cycle courses and study programmes. The research topic is “Biomedical science” and the Faculty examine Doctors of Philosophy (i.e., in Swedish: Teknologie doctor, Doktor i medicinsk vetenskap and Medicine doctor).
The doctoral students will have the opportunity to take part in teaching at undergraduate level, however, according to regulations, teaching and administrative work is limited to a maximum of 20% of working hours. Provisions on appointment as a doctoral student are laid out in the Swedish Higher Education Ordinance, Chapter 5, Sections 1-7.
When making the appointment, under Chapter 5, Section 5 of the Swedish Higher Education Ordinance, consideration must be given to the student´s ability to benefit from doctoral studies.
Project.The core question for the overall project is “How to achieve topical quantification of low molecular weight endogenous compounds for monitoring skin-disorder progression and healing?” To succeed with this enterprise Malmö University has teamed up with seven industry partners; Chemotechnique Diagnostics AB, Bioglan AB, in Vitro Plant-tech AB, ImaGene-iT AB, Medibiome AB, Scibase AB and Chromalytica AB, who share a genuine interest in skin related applications. Thus, the results of the project will have an immediate impact in industry and quicker return of the investment for modernizing healthcare.
A limiting factor in the development of non-invasive methods for detection of skin disorders is the restricted access to skin disease biomarkers. Biopsies are used today because the diagnostic decision is based on analysis of high molecular weight (HMW) biomarkers, e.g., cytokines or cell surface receptors. Since HMW biomarkers do not leak to the skin surface, these biomarkers cannot be used for non-invasive detection. However, skin barrier allows diffusion of low molecular weight (LMW) compounds (< 500 Da). Thus, we hypothesize that it is feasible to achieve robust non-invasive or minimally invasive monitoring of skin disorders by monitoring LMW biomarkers. Particularly, we aim to develop non-invasive or minimally invasive monitoring of LMW biomarkers of inflammation and cancer. The proposed approach will be studied using skin membranes in-vitro and validated using cell cultures. Highly specific assays for LMW biomarkers in skin-derived cells will be developed. We will assess in vitro-cultured skin cells for LMW biomarker generation and HMW biomarker expression by using HPLC-MS, fluorescent microscopy, flow cytometry, and ELISA. By comparing, HMW biomarker expression vs LMW biomarker generation the LMW biomarker approach to monitor skin disorders will be validated. We will also analyze the influence of different components in the plant extracts on inflammatory and cancer development processes in cell cultures.
Tasks. The skin forms the interface between the interior of the body and the exterior environment and, consequently, separates two extremely different regions. The candidate for this PhD position is expected to focus on how the skin barrier responds to changes in one or several gradients as they vary in accordance to fluctuations of the outside environment.
The general aim of this part of the project is to achieve accelerated and controlled hydration of skin to reach steady state fast, followed by monitoring of at least two related LMW biomarkers and accounting for how the LMW biomarker ratio on the skin surface reflects their ratio in viable tissue. More specifically the research questions envisioned comprise i) How can we change water sorption/desorption rates in skin – kinetics and hysteresis? ii) What is the effect of hydration on biomarkers distribution and penetration through skin? iii) Can hollow microneedles be used to avoid changes of biomarkers concentrations ratios that may occur during diffusion? and iv) What is the effect of patch excipients on penetration of biomarkers through skin?
Candidates must have a background in chemistry or pharmacy with a good understanding in thermodynamics. Experience from working with biological material, as well as in design and physicochemical characterization of lipid-based pharmaceutical formulations is advantageous. Special considerations will be given to previous hands-on experience in more than one of the following methods: microscopic techniques (e.g. PLOM, TPM, CLSM, TEM, SEM), X-ray diffraction and nanotomography, thermal analysis (ITC, TGA, DSC, ISC), in vitro drug release methodology (type Franz, USP Dissolutest). Documented excellent level of English (understanding, speaking and writing) is essential. The candidate is expected to present the results in form of manuscripts, and should also have the ability to work in a team and collaborate with people in complementary disciplines.
Apart from carrying out research within the mentioned areas, the applicant is expected to take responsibility for the maintenance of relevant instruments and participate in teaching
Form of employment and starting date
Temporary position, starting September 2017
Johan Engblom, Professor in Biomedical technology
Phone: +46 40 665 74 83, Mail: firstname.lastname@example.org
Tautgirdas Ruzgas, Professor in Biomedical technology
Phone: +46 40 665 74 31, Mail: email@example.com
Anette Gjörloff Wingren, Professor in biomedical laboratory science
Phone: +46 70 6011857, Mail: firstname.lastname@example.org
Instructions to Applicants
You apply through Malmö University’s recruitment system by clicking the button “ansök”. It is your own responsibility that your application is complete with all required documentation according to the adverted position. Your application must have arrived no later than August 15th, 2017.
The application should contain the following:
- A brief summary of scientific, teaching and other qualifications relevant to the position, together with a motivation for applying to this PhD-student position
- Attested copies of certificates
- Signed list of qualifications (CV)
- List of scientific publications
- Copies of three of your most relevant scientific publications
Trade union representatives
SACO, Ann-Mari Campbell tel: 040-665 74 37
OFR/Lärarförbundet, Peter Persson tel:040-665 82 76
OFR/S (ST) Naser Eftekharian tel: 040-665 76 49